# N and O isotope effects of nitrate reductases # PI: Julie Granger (UConn) # Version: 12 Aug 2015 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 10 11082014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 10.0 915.4 24.7 0.0 18.7 0.0 10.1 880.9 29.5 0.0 23.7 0.0 10.2 736.8 35.3 0.0 29.6 0.1 10.3 407.3 54.9 0.1 48.3 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 11 11082014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 11.0 873.2 27.8 0.0 21.8 0.0 11.1 772.5 30.7 0.1 24.3 0.0 11.2 548.4 39.7 0.2 33.1 0.1 11.3 507.8 45.4 0.0 38.4 0.1 11.4 310.5 59.9 0.1 52.0 0.0 11.5 181.3 72.2 0.1 63.0 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 12 11082014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 12.0 904.2 24.2 0.0 18.5 0.1 12.1 780.3 28.8 0.0 23.2 0.0 12.2 705.1 32.5 0.1 26.9 0.1 12.3 618.4 37.5 0.4 31.7 0.0 12.4 431.4 49.6 0.1 43.1 0.1 12.5 313.6 57.4 0.2 50.4 0.2 12.6 234.0 66.3 0.3 58.9 0.3 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 13 11082014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 13.0 888.6 24.3 0.1 18.7 0.2 13.1 847.5 27.4 0.0 21.9 0.0 13.2 757.9 30.8 0.1 24.8 0.0 13.3 669.6 34.2 0.2 28.0 0.1 13.4 546.4 40.9 0.0 34.7 0.1 13.5 464.3 46.0 0.1 39.4 0.0 13.6 385.9 51.9 0.1 45.2 0.1 13.7 234.2 66.8 0.2 59.3 0.3